isotopes of copper

Progression of symptoms caused by Botryosphaeria dothidea on apple branches

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Progression of symptoms caused by Botryosphaeria dothidea on apple branches

Until lately, the causal agent of Botryosphaeria canker was assumed to vary from that inflicting ring rot on fruit and warts on branches on apple bushes in China and East Asia. However, latest analysis documented that Botryosphaeria dothidea caused each illness symptoms on apple. Inoculations with strains remoted from cankers and warts on branches have been carried out to research symptom development caused by B. dothidea and circumstances inducing the 2 symptom sorts. The outcomes confirmed that each cankers and warts are caused by B. dothidea.

Warts are the outcomes of hyperplasia and suberization of bark tissues induced by fungal an infection, whereas cankers end result from the speedy progress of hyphae from inside warts, lenticels or wounds. Resistance to B. dothidea exists in dwelling apple branches. When a dwelling department is contaminated by way of lenticels, the pathogen induces proliferation and suberization of cortical cells that restricts the expansion and enlargement of the hyphae, resulting in wart symptom. However, below sure stress circumstances resembling drought, the hyphae inside host tissues broaden quickly and kill cortical cells, resulting in canker improvement.

Host resistance might get well throughout energetic progress durations, which suppresses and even stops speedy enlargement of the hyphae, resulting in the intermediate symptom of canker warts. Abiotic elements, resembling drought or excessive temperature in early spring, can lead to speedy extension of colonized hyphae in branches and conversion of warts to cankers. Preventing this transition will be an essential measure in managing Botryosphaeria canker on apple.

Predictive Modeling of Brown Rust of Sugarcane Based on Temperature and Relative Humidity in Florida

Logistic regression fashions have been developed from 5 years (2014-2018) of illness severity and climate information in an try and predict brown rust of sugarcane on the Everglades Research and Education Center (EREC) in Belle Glade, Florida. Disease severity (proportion space of the highest seen dewlap leaf lined by rust) was visually assessed within the subject each two weeks for 2 varieties inclined to brown rust.

Two hundred fifty variables have been derived from climate information for 10 to 40-day durations earlier than every brown rust evaluation day. A subset of these variables have been then evaluated as potential predictors of severity of brown rust primarily based on their particular person correlation and/or their organic meaningfulness. The prediction accuracy of these fashions ranged from 73-85%. Single variable mannequin BR2 (primarily based on AHTR) labeled 89% of the epidemic and 81% of the non-epidemic standing of the illness.

Analyses of correlation and stepwise logistic regression allowed us to determine afternoon humid thermal ratio (AHTR), temperature-based period variables, and their interplay phrases as essentially the most vital variables related to brown rust epidemics of sugarcane in Florida. The 9 finest predictive fashions have been recognized primarily based on mannequin accuracy, sensitivity, specificity and estimates of the prediction error. Greater than 83% of the epidemics and 81% of the non-epidemic standing of sugarcane brown rust was accurately labeled utilizing a number of variable fashions. These fashions can doubtlessly be used as elements of a rust illness warning system to help within the administration of brown rust epidemics of sugarcane in south Florida.

Severe acute respiratory syndrome coronavirus 2 causes direct injury to the airway epithelium, enabling aspergillus invasion. Reports of COVID-19-associated pulmonary aspergillosis have raised considerations about it worsening the illness course of COVID-19 and growing mortality. Additionally, the primary instances of COVID-19-associated pulmonary aspergillosis caused by azole-resistant aspergillus have been reported.

This article constitutes a consensus assertion on defining and managing COVID-19-associated pulmonary aspergillosis, ready by consultants and endorsed by medical mycology societies. COVID-19-associated pulmonary aspergillosis is proposed to be outlined as doable, possible, or confirmed on the premise of pattern validity and thus diagnostic certainty. Recommended first-line remedy is both voriconazole or isavuconazole. If azole resistance is a priority, then liposomal amphotericin B is the drug of alternative. Our intention is to supply definitions for medical analysis and up-to-date suggestions for medical administration of the prognosis and therapy of COVID-19-associated pulmonary aspergillosis.

Progression of symptoms caused by Botryosphaeria dothidea on apple branches

From Hashtag to High School: How Viral Tweets Are Inspiring Young Scientists To Embrace STEM

Social media is an more and more essential skilled software for scientists. In explicit, scientists use their social media profiles to speak science and construct communities with like-minded scientists and nonscientists. These networks embrace journalists who can amplify social media science communication, disseminating it to new audiences on- and offline. Our expertise with an outreach challenge the place Peeps marshmallows have been inoculated with various fungi, which we referred to as #FungalPeeps, has demonstrated that these networks will be an efficient conduit between analysisers and highschool college students.

Dual Luciferase Reporter Assay Kit
DL101-01 100 rxn
EUR 90.5

Dual Luciferase Reporter Assay Kit
DL101-01-100rxns 100 rxns
EUR 93.71

Single-Luciferase Reporter Assay Kit
abx098133-100l 100 µl
EUR 650

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abx098133-1ml 1 ml Ask for price

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abx098133-200l 200 µl
EUR 762.5

Single-Luciferase Reporter Assay Kit
20-abx098133
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  • 50 rxns
  • 200 rxns

Double-Luciferase Reporter Assay Kit
abx098134-100l 100 µl
EUR 687.5

Double-Luciferase Reporter Assay Kit
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abx098134-200l 200 µl
EUR 900

Double-Luciferase Reporter Assay Kit
20-abx098134
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  • 50 rxns
  • 200 rxns

PDE1B Cell-Based Reporter Assay Kit
GWB-PS8329 500reactions Ask for price

Luciferase Reporter Gene Assay Kit
Z5030001 200 assays
EUR 329

Luciferase Reporter Gene Assay Kit
Z5030002 500 assays
EUR 636

Luciferase Reporter Gene Assay Kit
Z5030003 1,000 assays
EUR 1118

Single-Luciferase (Renilla) Reporter Assay Kit
abx298011-100g 100 µg Ask for price

Single-Luciferase (Renilla) Reporter Assay Kit
20-abx298011
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  • Ask for price
  • 50 rxns
  • 200 rxns

Single-Luciferase (Renilla) Reporter Assay Kit
abx298011-20g 20 µg
EUR 675

Single-Luciferase (Renilla) Reporter Assay Kit
abx298011-50g 50 µg
EUR 850

Dual Luciferase Reporter Gene Assay Kit
KTA8010-1000T 1000 T
EUR 819

Dual Luciferase Reporter Gene Assay Kit
KTA8010-100T 100 T
EUR 149

Dual Luciferase Reporter Gene Assay Kit
KTA8010-each each Ask for price

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Firefly Luciferase Reporter Gene Assay Kit
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Renilla Luciferase Reporter Gene Assay Kit
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RG021M 1000 Tests Ask for price

Gaussia Luciferase Reporter Gene Assay Kit
RG021S 100 Tests Ask for price

Notch Signaling Pathway Notch1/CSL Reporter - HEK293 Cell Line
60652 2 vials
EUR 2175
Description: The Notch CSL Reporter - HEK293 cell line contains the firefly luciferase gene under the control of Notch-response elements (CSL responsive elements) alone with expression construct for Notch1 E (NOTCH1 that has a deletion of the entire extracellular domain) stably integrated into HEK293 cells. Inside the cells, the Notch1 E can be cleaved by γ-secretase. The active Notch1 NICD is released to nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter using a known inhibitor of the Notch signaling pathway.

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RG029M 1000 Tests Ask for price

Dual Luciferase Reporter Gene Assay Kit II
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Firefly Luciferase Reporter Gene Assay Kit II
RG007M 1000 Tests Ask for price

Firefly Luciferase Reporter Gene Assay Kit II
RG007S 100 Tests Ask for price

RARα Reporter Cellular Assay Pack
79322 2 vials
EUR 2390
Description: The RARα Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor alpha (RARα). The pack contains the RARα Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. NM_000964). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARα is confirmed by Western blotting._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

RARβ Reporter Cellular Assay Pack
79323 2 vials
EUR 2050
Description: The RARβ Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor beta (RARβ). The pack contains the RARβ Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. P10826-2). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARβ is confirmed by Western blotting._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

RARγ Reporter Cellular Assay Pack
79324 2 vials
EUR 2050
Description: The RARγ Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor gamma (RARγ). The pack contains the RARγ Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. P13631-1). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARγ is confirmed by Western blotting._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

Dual-LumiTM Luciferase Reporter Gene Assay Kit
RG088M 1000 Tests Ask for price

Dual-LumiTM Luciferase Reporter Gene Assay Kit
RG088S 100 Tests Ask for price

Single Firefly Luciferase Reporter Gene Assay Kit
K2236-100 100T
EUR 68
Description: Transcriptional Regulation|Reporter Gene

Single Firefly Luciferase Reporter Gene Assay Kit
K2236-1000 1000T
EUR 336
Description: Transcriptional Regulation|Reporter Gene

Human Notch Signaling Pathway Notch1/CSL Reporter Cell Line-HEK293
ABC-RC0116 1 vial Ask for price
Description: Human Notch Signaling Pathway Notch1/CSL Reporter Cell Line is derived from HEK293. Application: • Monitor Notch signaling pathway activity.• Screen for activators or inhibitors of the Notch signaling pathway.

Enhanced Firefly Luciferase Reporter Gene Assay Kit
RG009M 1000 Tests Ask for price

Enhanced Firefly Luciferase Reporter Gene Assay Kit
RG009S 100 Tests Ask for price

Renilla-LumiTM Luciferase Reporter Gene Assay Kit
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Renilla-LumiTM Luciferase Reporter Gene Assay Kit
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Enhanced Firefly Luciferase Reporter Gene Assay Kit II
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Enhanced Firefly Luciferase Reporter Gene Assay Kit II
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Bio-LumiTM Firefly Luciferase Reporter Gene Assay Kit
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Bio-LumiTM Firefly Luciferase Reporter Gene Assay Kit
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One-LumiTM Firefly Luciferase Reporter Gene Assay Kit
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One-LumiTM Firefly Luciferase Reporter Gene Assay Kit
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Notch1 NICD Pathway Reporter Kit (Mouse)
79504 500 rxns.
EUR 590
Description: Notch Pathway Reporter kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for Mouse NOTCH1 that has a deletion of the entire extracellular domain and transmembrane region (NOTCH1 NICD). Inside the cells, the NOTCH1 NICD is constitutively localized into the nucleus without needing to be cleaved by γ-secretase. The kit also contains CSL (CBF1/RBP-Jκ) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jκ) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_This kit contains the expression vector for Mouse Notch1 NICD, which does not require γ-secretase processing to become active. We also offer the Mouse Notch1DE expression vector (BPS Bioscience #60509), as well as a Human Notch1DE expression vector (BPS Bioscience #79503), which are dependent on γ-secretase activity to become active.

Renilla-LumiTM Plus Luciferase Reporter Gene Assay Kit
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Bright-LumiTM Firefly Luciferase Reporter Gene Assay Kit
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NF-κB Reporter Cellular Assay Pack (CHOK1)
79325 2 vials
EUR 1245
Description: The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)- CHO-K1 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine. _x000D_Additionally, the pack includes cell culture medium (Thaw Medium 3) that has been optimized for use with CHO-K1 cells. Thaw Medium 3 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
12518-1plate 1 plate
EUR 166
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
12519-10plates 10 plates
EUR 446
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
12520-100plates 100 plates
EUR 3321
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Bio-LumiTM II Firefly Luciferase Reporter Gene Assay Kit
RG043M 1000 Tests Ask for price

Bio-LumiTM II Firefly Luciferase Reporter Gene Assay Kit
RG043S 100 Tests Ask for price

NF-κB Reporter Cellular Assay Pack (HEK293)
79327 2 vials
EUR 1515
Description: The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)-HEK293 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 1) that has been optimized for use with HEK293 cells. Thaw Medium 1 includes 10% fetal bovine serum, non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_

Bio-LumiTM One Firefly Luciferase Reporter Gene Assay Kit
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Bio-LumiTM Steady Firefly Luciferase Reporter Gene Assay Kit
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Bio-LumiTM One II Firefly Luciferase Reporter Gene Assay Kit
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Bac-LumiTM Bacterial Firefly Luciferase Reporter Gene Assay Kit
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Dual-LumiTM II Luciferase Reporter Gene Assay Kit, Dual-LumiTM II Luciferase Assay Kit
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Bio-LumiTM Steady II Firefly Luciferase Reporter Gene Assay Kit
RG047M 1000 Tests Ask for price

Bio-LumiTM Steady II Firefly Luciferase Reporter Gene Assay Kit
RG047S 100 Tests Ask for price

Amplite® Luciferase Reporter Gene Assay Kit *Maximized Luminescence*
12519 10 plates
EUR 455
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12530-1plate 1 plate
EUR 222
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12531-10Plates 10 Plates
EUR 846
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12532-100plates 100 plates
EUR 4447
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12535-1plate 1 plate
EUR 222
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12536-10plates 10 plates
EUR 1070
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12537-100plates 100 plates
EUR 4447
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Dual Luciferase Reporter Gene Assay Cell Lysis Buffer
RG132M 100 mL Ask for price

Dual Luciferase Reporter Gene Assay Cell Lysis Buffer
RG132S 10 mL Ask for price

Firefly Luciferase Reporter Gene Assay Cell Lysis Buffer
RG126M 100 mL Ask for price

Firefly Luciferase Reporter Gene Assay Cell Lysis Buffer
RG126S 10 mL Ask for price

Renilla Luciferase Reporter Gene Assay Cell Lysis Buffer
RG129M 100 mL Ask for price

Renilla Luciferase Reporter Gene Assay Cell Lysis Buffer
RG129S 10 mL Ask for price

Gaussia Luciferase Reporter Gene Assay Cell Lysis Buffer
RG135M 100 mL Ask for price

Gaussia Luciferase Reporter Gene Assay Cell Lysis Buffer
RG135S 10 mL Ask for price

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Maximized Luminescence*
12536 10 plates
EUR 1092
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

One-LumiTM II Firefly Luciferase Reporter Gene Assay Kit, One-LumiTM II Firefly Luciferase Assay Kit
RG056M 1000 Tests Ask for price

One-LumiTM II Firefly Luciferase Reporter Gene Assay Kit, One-LumiTM II Firefly Luciferase Assay Kit
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Bright-LumiTM II Firefly Luciferase Reporter Gene Assay Kit, Bright-LumiTM II Firefly Luciferase Assay Kit
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Steady-LumiTM II Firefly Luciferase Reporter Gene Assay Kit, Steady-LumiTM II Firefly Luciferase Assay Kit
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Enhanced Firefly Luciferase Reporter Gene Assay Cell Lysis Buffer
RG127M 100 mL Ask for price

Enhanced Firefly Luciferase Reporter Gene Assay Cell Lysis Buffer
RG127S 10 mL Ask for price

Dual Notch1-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1) Cell Line
T3032 1x10^6 cells / 1.0 ml
EUR 3950

CRE/CRE Reporter Kit
GWB-PS935F 500reactions Ask for price

TCF Reporter Plasmid (TOPflash, TK-luciferase reporter)
MBS641020-0005mg 0.005mg
EUR 1270

TCF Reporter Plasmid (TOPflash, TK-luciferase reporter)
MBS641020-5x0005mg 5x0.005mg
EUR 5570

WNT TCF/LEF reporter kit
GWB-PS656B 500rxns. Ask for price

Dual Notch1(Variant)-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1G4<sup>esn</sup>) Cell Line
T3033 1x10^6 cells / 1.0 ml
EUR 3950

AP1 Reporter Kit (JNK Pathway)
60612 500 rxns.
EUR 565
Description: The AP1 Reporter Kit is designed for monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured cells. The kit contains a transfection-ready AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.

AP1 Reporter Kit (JNK Pathway)
GWB-PSB7DB 500reactions Ask for price

7TFP CDH1 reporter
PVT34088 2ug
EUR 280

Following standard science journalism, #FungalPeeps, a challenge initiated at West Virginia University, impressed a mycology analysis challenge in Notre Dame High School in San Jose, California. Herein, we describe how this connection between academia, journalists, and the highschool classroom occurred, and the way everybody concerned benefited from this academic collaboration. We additional counsel ways in which fashionable social media networks could possibly be leveraged to include extra such sensible studying experiences into progressive science curricula to higher domesticate younger STEM scientists.